recombinant human gas6 protein  (R&D Systems)

Journal: Turkish Journal of Obstetrics and Gynecology

Article Title: Evaluating midpregnancy Gas6 levels as predictive value for gestational diabetes and birth outcomes

doi: 10.4274/tjod.galenos.2025.95515

Figure Lengend Snippet: ROC curve for the diagnosis of gestational diabetes mellitus based on serum Gas6 levels GAS6: Growth arrest–specific 6 protein, ROC: Receiver operating characteristic

Article Snippet: Gas6 levels were measured using a double antibody sandwich method with a human Gas6 ELISA kit, branded BT Lab Bioassay Technology Laboratory (Cat No. E3257Hu, Shanghai, China).

Techniques: Biomarker Discovery

Journal: Turkish Journal of Obstetrics and Gynecology

Article Title: Evaluating midpregnancy Gas6 levels as predictive value for gestational diabetes and birth outcomes

doi: 10.4274/tjod.galenos.2025.95515

Figure Lengend Snippet:

Article Snippet: Gas6 levels were measured using a double antibody sandwich method with a human Gas6 ELISA kit, branded BT Lab Bioassay Technology Laboratory (Cat No. E3257Hu, Shanghai, China).

Techniques: Biomarker Discovery

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: (A) Binding of different antibodies to AXL. Binding of different antibodies to AXL and their interaction with Gas6. (B) List of full length and different truncations of AXL. (C) αAXL-9# and αAXL-13# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations. (D) αAXL-4# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations with or without Gas6-his.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Binding Assay, Flow Cytometry

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: The binding characteristic of AXL-4#. (A) Representation of interplay of AXL antibody and Gas6. (B) 293T-hAXL cells were incubated with isotype control (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (C) 293T-memGas6 cells were incubated with anti-Gas6l (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (D,E) The structure of αAXL-4# recognizing Gas6/AXL complex predicted by AlphaFold.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Binding Assay, Incubation, Control, Staining

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Application of αAXL-4# in flow cytometry. (A,B) The binding of αAXL-4# to Gas6/AXL complex of different Species. (C,D) asDCs and other cell lines were incubated with αAXL-9#, αAXL-13 and αAXL-4#.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Flow Cytometry, Binding Assay, Incubation

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Application of αAXL-4# in ELISA. (A) Different concentrations of AXL (0-1 μg/mL) with a single dose of Gas6 (0.25 μg/mL, red column) or without Gas6 (empty column) were added subsequently. (B) Different concentrations of Gas6 (0–1 μg/mL) with a single dose of AXL (1 μg/mL, red column) or without AXL (empty column) were added subsequently.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Different levels of AXL-Gas6, AXL and Gas6 in human plasma. (A–C) Plates were coated by AXL-4# (A) , commercial AXL antibody (B) or commercial Gas6 antibody (C) respectively. The levels of AXL-Gas6, AXL, or Gas6 in human plasma were detected by ELISA.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: ROC curve and association with AXL/AXL-Gas6 with clinical data. (A) ROC curve; (B) AXL was associated with Cr in STEMI; (C–G) Gas6/AXL complex was associated with PLT, WBC, HDL, CK-MB and SO-FMC as indicated.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques:

Journal: bioRxiv

Article Title: EndoNB: A general strategy to study the internalization of cell surface proteins

doi: 10.1101/2025.06.08.658482

Figure Lengend Snippet: a , Structural alignment of the hybrid and β-I domains of wild-type and Alfa-tagged Integrin β1. b , Schematics showing the effect of 12G10 and Mab13 antibodies on integrin β1 activation state. These antibodies act by stabilizing the integrins in their active or inactive state. c , Quantification of integrin β1 uptake by flow cytometry using EndoNB in the presence of no antibodies (no treatment), or in the presence of 12G10 (activating), Mab13 (inactivating) or unrelated (anti integrin αvβ3 antibody). (n = 12, each n represents the median fluorescence of 2000-8000 cells). d , Structural alignment of the extracellular region of wild-type and Alfa-tagged transferrin receptor (TFR). Wild type: PDB 1suv, while Alfa-tagged was generated by AlphaFold 3. See for comparison of Alfa-tagged dimeric and monomeric TFR. e , Schematics showing the experimental design to test if transferrin receptor uptake is stimulated by the presence of transferrin. f , Quantification of transferrin receptor uptake for 15 or 30 minutes by flow cytometry using EndoNB in the presence of increasing concentrations of transferrin. (n = 4, each n represents the median fluorescence of 2000-8000 cells). See for quantification of transferrin-AlexaFluor488 signals at each condition. g , Structural alignment of the transmembrane and extracellular region of wild-type and Alfa-tagged AXL. Both structures generated with AlphaFold 3. h , Quantification of AXL uptake by flow cytometry using EndoNB in the absence or presence of the AXL ligand GAS6 (1ug/ml). (n = 4, each n represents the median fluorescence of 2000-8000 cells). See for comparison with the conditions with and without 3C. ns = non-significant, * p> 0.05, **** p> 0.0001. ANOVA with Tukey’s post hoc analysis.

Article Snippet: SNAP-JF646 substrate was a kind gift by Luke Lavis via the open chemistry team (jJanelia); Transferrin-Alexa Fluor 488 (Thermo Fisher scientific, #T13342); Phalloidin-iFluor 488 (AAT Bioquest, #23115); GAS6 recombinant protein (MedChemExpress via Nordic Biosite HY-P700724-20).

Techniques: Activation Assay, Flow Cytometry, Fluorescence, Generated, Comparison

Journal: bioRxiv

Article Title: EndoNB: A general strategy to study the internalization of cell surface proteins

doi: 10.1101/2025.06.08.658482

Figure Lengend Snippet: a , Structural alignment of the monomeric and dimeric extracellular region of wild-type and Alfa-tagged transferrin receptor (TFR, PDB). Wild type: PDB 1suv, while Alfa-tagged was generated by AlphaFold 3. b , Quantification of transferrin (AlexaFluor488) in each condition measured in . c , Quantification of AXL uptake by flow cytometry using EndoNB in the absence or presence of the AXL ligand GAS6 (1µg/ml) and also in the presence or not of 3C. (n = 4, each n represents the median fluorescence of 2000-8000 cells). ns = non-significant, ** p> 0.01, *** p> 0.001, **** p> 0.0001. ANOVA with Tukey’s post hoc analysis.

Article Snippet: SNAP-JF646 substrate was a kind gift by Luke Lavis via the open chemistry team (jJanelia); Transferrin-Alexa Fluor 488 (Thermo Fisher scientific, #T13342); Phalloidin-iFluor 488 (AAT Bioquest, #23115); GAS6 recombinant protein (MedChemExpress via Nordic Biosite HY-P700724-20).

Techniques: Generated, Flow Cytometry, Fluorescence

Journal: Stem Cell Research & Therapy

Article Title: Combination of rapamycin and adipose-derived mesenchymal stromal cells enhances therapeutic potential for osteoarthritis

doi: 10.1186/s13287-024-04090-8

Figure Lengend Snippet: Rapamycin induces the secretion of chondroprotective and regenerative factors by AD-MSCs. A The list of rapamycin-upregulated genes ( vs vehicle) in AD-MSCs was submitted to KEGG Pathway . Significantly affected pathways (at FDR ≤ 0.05) were plotted as a bubble chart. B The KEGG network of “Hedgehog signaling” and “TGF-β signaling” upregulated genes identified in panel A. C Genes encoding extracellular factors and upregulated or downregulated in AD-MSCs were selected using the Gene Ontology term “Extracellular region ” (GO0005576) and plotted as a Z-score heatmap of normalized counts showing the vehicle- vs. rapamycin-treated groups. D Transcripts Per Million (TPM) of extracellular factors from the “Hedgehog signaling” (SCUBE2 and MEGF8 ) and “TGF-β signaling” ( LTBP2 and FST ) pathways were extracted and plotted as dot-histograms to show their relative expression in the indicated conditions. E TPM of the pro-regenerative factor GAS6 . F GAS6 secreted by AD-MSCs upon exposure to rapamycin or/and IFN-γ. GAS6 level in supernatants was quantified by ELISA and normalized to the relative number of cells; G Diagram summary of all results. p -values: * < 0.05, ** < 0.01, *** < 0.001 (two-way ANOVA)

Article Snippet: GAS6 secretion by AD-MSCs was assessed using the Human GAS6 ELISA Kit (Invitrogen – BMS2291) following the manufacturer’s instruction, but by initially diluting samples five times in diluent buffer (see Supplementary data for details).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Spatio-temporal dynamics of the fibrotic niche in cardiac repair

doi: 10.1101/2024.11.10.622609

Figure Lengend Snippet: A , Spatial maps showing MP and FB subtypes in the wound (LV edge in sham). B , day 7 neighborhoods surrounding Postn+Thbs4+ myoFB, with MP highlighted in blue. C , spatial gene factor co-variations of MP neighborhood in day 7 heart. Circle size scales linearly with −log 10 (p-value), and circle color indicates ridge regression coefficients. D , genes positively and negatively associated with gene factor 3 (Fa3) of MP and Fa2 of FB, respectively. Highlighted genes in MP-positive-Fa3: secreted ligands; MP-negative-Fa3: early proinflammatory/IFN pathway; FB-positive-Fa2: ECM genes, FB-negative-Fa2: cell cycle. E , pathways negatively associated with FB Fa2 (BioPlanet 2019 database). F , FB UMAP with inferred cell fate transition and cell cycle G2M gene score (upper), and time points post-lesion. G , boxplot comparing log2-transformed aggregated G2M gene expression of FB over time. H , CellChat ligand-receptor interaction prediction between MP (signal senders) and FB subtypes (signal receivers) across day 3–28. I , immune cell UMAP showing Gas6 and Pros1 expression, and FB UMAP showing Axl expression. J , multiplexed-IF staining of day 7 post-LAD IZ. Dotted lines highlight CD45+ GAS6+ PROS1+ immune cells contacting myoFB (CD140a+). K – M , mouse cardiac myoFB culturing experiment (Methods). Cultured myoFB were harvested for IF staining with αSMA and Mki67 ( K ), quantification of % Mki67+ nuclei (DAPI+) in αSMA+ cells (myoFB) ( l ), as well as qRT-PCR quantification of senescence marker gene Glb1 (β-gal) ( M ). N , CellChat prediction for FB (senders) and MP subtypes (receivers), across day 3-28. O , UMAPs of FB (left) and immune (right) clusters plotting SEMA3 family and their receptor’s gene expression. P and Q , mouse BMDM culturing. BMDM were exposed to BSA/SEMA3D for 72 hours, then harvested for H3P IF ( P ) with intensity quantification ( Q ). For ( L ), ( M ), ( P ), ( Q ), statistical significance was calculated by unpaired two-tailed t-test for two experimental groups. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; ns, not significant.

Article Snippet: Cells were washed twice with PBS, and then incubated with either serum-free DMEM or full DMEM medium, with the addition of Recombinant Human Gas6 protein (R&D Systems, 885-GSB-050) or the equivalent amount of BSA.

Techniques: Transformation Assay, Expressing, Staining, Cell Culture, Quantitative RT-PCR, Marker, IF-P, Two Tailed Test

Journal: Viruses

Article Title: Nano-Biomechanical Investigation of Phosphatidylserine-Mediated Ebola Viral Attachment via Human Gas6 and Axl

doi: 10.3390/v16111700

Figure Lengend Snippet: Characterization results of Gas6 interacting with Axl or PS on Ebola VLP. ( A ) Single molecule force measurement data are analyzed using the Bell–Evans model and the fitting results describe the Gas6–Axl (n = 451) and Gas6–VLP (n = 260) interactions as a relation between the unbinding force and loading rate. The fitting results also provide dissociation rates ( k 0 ) and reaction lengths ( γ ) of these two interactions to evaluate their binding affinity and bound complex energy barrier, which are listed as well. ( B ) Microscale thermophoresis (MST) analysis of the interaction between Gas6 and Axl (n = 7). The fitting result is used to determine the binding affinity in the form of dissociation constant (Kd). ( C ) The binding frequency comparison is used to show the interaction specificity between experiment groups and control groups. The binding frequency measurements are all conducted under the same conditions. All given error bars show the standard deviation. Significance is determined by an unpaired t -test. *: <0.05; **: <0.01; ns: not significant. n is the total sample number used for analysis.

Article Snippet: Human Gas6 protein was purchased from R&D systems (Minneapolis, MN, USA, catalog #885-GSB) and was expressed from mouse myeloma cell NS0, which contains AA Ala 49-Trp 678 (Accession #NP_000811).

Techniques: Binding Assay, Microscale Thermophoresis, Comparison, Control, Standard Deviation

Journal: Viruses

Article Title: Nano-Biomechanical Investigation of Phosphatidylserine-Mediated Ebola Viral Attachment via Human Gas6 and Axl

doi: 10.3390/v16111700

Figure Lengend Snippet: ( A ) Scheme shows the niche and the instrument settings during the measurement of VLP–Gas6–Axl (n = 427). ( B ) Single molecule force measurement result comparison between Gas6–Axl (n = 451), Gas6–VLP (n = 260), and VLP–Gas6–Axl (n = 427). Dissociation rate ( k 0 ) and reaction length ( γ ) for these interactions are shown. ( C ) The binding frequency comparison is used to show the interaction specificity between experiment groups and control groups. The binding frequency measurements are all conducted under the same conditions. All given error bars show the standard deviation. Significance is determined by an unpaired t -test. *: <0.05; **: <0.01; ns: not significant. n is the total sample number used for analysis.

Article Snippet: Human Gas6 protein was purchased from R&D systems (Minneapolis, MN, USA, catalog #885-GSB) and was expressed from mouse myeloma cell NS0, which contains AA Ala 49-Trp 678 (Accession #NP_000811).

Techniques: Comparison, Binding Assay, Control, Standard Deviation

Journal: Viruses

Article Title: Nano-Biomechanical Investigation of Phosphatidylserine-Mediated Ebola Viral Attachment via Human Gas6 and Axl

doi: 10.3390/v16111700

Figure Lengend Snippet: Single molecule force measurement result comparison to reveal the impact of calcium ion on interactions among VLP, Gas6, and Axl. ( A ) VLP–Gas6 interaction with (blue dashed line, n = 308) and without (blue solid line, n = 260) calcium ions present and their binding frequency comparison to examine the interaction specificity ( B ) VLP–Gas6–Axl interaction with (black dashed line, n = 397) and without (black solid line, n = 427) calcium ions present and their binding frequency comparison to examine the interaction specificity. ( C ) Gas6–Axl interaction with (red dashed line, n = 341) and without (red solid line, n = 451) calcium ions present and their binding frequency comparison to examine the interaction specificity. The binding frequency comparison is used to show the interaction specificity between experiment groups and control groups. The binding frequency measurements are all conducted under the same conditions. All given error bars show the standard deviation. Significance is determined by an unpaired t -test. *: <0.05; **: <0.01; ns: not significant. n is the total sample number used for analysis.

Article Snippet: Human Gas6 protein was purchased from R&D systems (Minneapolis, MN, USA, catalog #885-GSB) and was expressed from mouse myeloma cell NS0, which contains AA Ala 49-Trp 678 (Accession #NP_000811).

Techniques: Comparison, Binding Assay, Control, Standard Deviation

Journal: Viruses

Article Title: Nano-Biomechanical Investigation of Phosphatidylserine-Mediated Ebola Viral Attachment via Human Gas6 and Axl

doi: 10.3390/v16111700

Figure Lengend Snippet: Comparison and summary of all single molecule force measurement results in this study to reveal the binding mechanism among VLP, Gas6, and Axl biomechanically and the impact of calcium ions on binding strengths and binding affinities.

Article Snippet: Human Gas6 protein was purchased from R&D systems (Minneapolis, MN, USA, catalog #885-GSB) and was expressed from mouse myeloma cell NS0, which contains AA Ala 49-Trp 678 (Accession #NP_000811).

Techniques: Comparison, Binding Assay